How to Calculate Peptide Dose After Reconstitution
The 3-step formula for any peptide, any syringe
4 min readQuick Answer:
After reconstitution, calculate your concentration (peptide mg / water ml), then divide your desired dose by that concentration to get draw volume. On a U-100 syringe, multiply ml by 100 to get units.

The 3 Formulas
Step 1: Calculate Concentration
Example: 5mg peptide in 2ml water = 2.5mg/ml
Step 2: Calculate Draw Volume
Example: 0.25mg / 2.5mg/ml = 0.1ml
Step 3: Convert to Syringe Units
U-40: Draw Volume (ml) x 40 = Units
Example (U-100): 0.1ml x 100 = 10 units
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Common Conversions
| mcg | mg | At 2.5mg/ml | U-100 Units |
|---|---|---|---|
| 100mcg | 0.1mg | 0.04ml | 4 units |
| 200mcg | 0.2mg | 0.08ml | 8 units |
| 250mcg | 0.25mg | 0.1ml | 10 units |
| 500mcg | 0.5mg | 0.2ml | 20 units |
| 1000mcg | 1mg | 0.4ml | 40 units |
Reverse Calculation
If you already drew a certain number of units and want to know the dose:
ml drawn x Concentration (mg/ml) = Dose (mg)
Example: Drew 12 units at 2.5mg/ml → 0.12ml x 2.5 = 0.3mg = 300mcg
Common Mistakes
- Confusing mcg and mg - 250mcg = 0.25mg. A 1,000x error will ruin your day.
- Wrong syringe type - U-40 and U-100 have different unit markings. 10 units on a U-40 is NOT the same volume as 10 units on a U-100.
- Forgetting reconstitution volume - If you used 1ml water instead of 2ml, your concentration is 2x higher. Recalculate.
Related Questions
- BPC-157 reconstitution: complete worked example
- How many doses are in a 5mg peptide vial?
- How long do reconstituted peptides last?
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See Plans & PricingDisclaimer: This is a calculation reference, not medical advice. Verify all calculations before use. Consult a healthcare provider for dosing guidance.